论文
论文标题：Molecular hallmarks of heterochronic parabiosis at single-cell resolution
作者：Pálovics, Róbert, Keller, Andreas, Schaum, Nicholas, Tan, Weilun, Fehlmann, Tobias, Borja, Michael, Kern, Fabian, Bonanno, Liana, Calcuttawala, Kruti, Webber, James, McGeever, Aaron, Luo, Jian, Pisco, Angela Oliveira, Karkanias, Jim, Neff, Norma F., Darmanis, Spyros, Quake, Stephen R., Wyss-Coray, Tony
期刊：Nature
发表时间：2022/03/02
数字识别码：10.1038/s41586-022-04461-2
摘要：The ability to slow or reverse biological ageing would have major implications for mitigating disease risk and maintaining vitality1. Although an increasing number of interventions show promise for rejuvenation2, their effectiveness on disparate cell types across the body and the molecular pathways susceptible to rejuvenation remain largely unexplored. Here we performed single-cell RNA sequencing on 20 organs to reveal cell-type-specific responses to young and aged blood in heterochronic parabiosis. Adipose mesenchymal stromal cells, haematopoietic stem cells and hepatocytes are among those cell types that are especially responsive. On the pathway level, young blood invokes new gene sets in addition to reversing established ageing patterns, with the global rescue of genes encoding electron transport chain subunits pinpointing a prominent role of mitochondrial function in parabiosis-mediated rejuvenation. We observed an almost universal loss of gene expression with age that is largely mimicked by parabiosis: aged blood reduces global gene expression, and young blood restores it in select cell types. Together, these data lay the groundwork for a systemic understanding of the interplay between blood-borne factors and cellular integrity.
2022年3月2日，美国斯坦福大学医学院Tony Wyss-Coray研究组、美国陈·扎克伯格医学中心Stephen R. Quake研究组以及Spyros Darmanis研究组、斯坦福大学医学院Andreas Keller研究组合作发文题为Molecular hallmarks of heterochronic parabiosis at single-cell resolution，揭开了异时异种共生过程中单细胞水平分子生物学标记物。
