论文
论文标题：SARS-CoV-2 immune evasion by the B.1.427/B.1.429 variant of concern
作者：Matthew McCallum , Jessica Bassi , Anna De Marco , Alex Chen , Alexandra C. Walls , Julia Di Iulio , M. Alejandra Tortorici , Mary-Jane Navarro , Chiara Silacci-Fregni , Christian Saliba , Kaitlin R. Sprouse , Maria Agostini , Dora Pinto , Katja Culap , Siro Bianchi , Stefano Jaconi , Elisabetta Cameroni , John E. Bowen , Sasha W Tilles , Matteo Samuele Pizzuto , Sonja Bernasconi Guastalla , Giovanni Bona , Alessandra Franzetti Pellanda , Christian Garzoni , Wesley C. Van Voorhis , Laura E. Rosen , Gyorgy Snell , Amalio Telenti , Herbert W. Virgin , Luca Piccoli , Davide Corti , David Veesler
期刊：Science
发表时间：2021/07/01
数字识别码：10.1126/science.abi7994
摘要：A novel variant of concern (VOC) named CAL.20C (B.1.427/B.1.429), originally detected in California, carries spike glycoprotein mutations S13I in the signal peptide, W152C in the N-terminal domain (NTD), and L452R in the receptor-binding domain (RBD). Plasma from individuals vaccinated with a Wuhan-1 isolate-based mRNA vaccine or convalescent individuals exhibited neutralizing titers, which were reduced 2-3.5 fold against the B.1.427/B.1.429 variant relative to wildtype pseudoviruses. The L452R mutation reduced neutralizing activity of 14 out of 34 RBD-specific monoclonal antibodies (mAbs). The S13I and W152C mutations resulted in total loss of neutralization for 10 out of 10 NTD-specific mAbs since the NTD antigenic supersite was remodeled by a shift of the signal peptide cleavage site and formation of a new disulphide bond, as revealed by mass spectrometry and structural studies.
