2021/07/02
论文
论文标题：Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants
作者：Lingshu Wang , Tongqing Zhou , Yi Zhang , Eun Sung Yang , Chaim A. Schramm , Wei Shi , Amarendra Pegu , Olamide K. Oloniniyi , Amy R. Henry , Samuel Darko , Sandeep R. Narpala , Christian Hatcher , David R. Martinez , Yaroslav Tsybovsky , Emily Phung , Olubukola M. Abiona , Avan Antia , Evan M. Cale , Lauren A. Chang , Misook Choe , Kizzmekia S. Corbett , Rachel L. Davis , Anthony T. DiPiazza , Ingelise J. Gordon , Sabrina Helmold Hait , Tandile Hermanus , Prudence Kgagudi , Farida Laboune , Kwanyee Leung , Tracy Liu , Rosemarie D. Mason , Alexandra F. Nazzari , Laura Novik , Sarah O’Connell , Sijy O’Dell , Adam S. Olia , Stephen D. Schmidt , Tyler Stephens , Christopher D. Stringham , Chloe Adrienna Talana , I-Ting Teng , Danielle A. Wagner , Alicia T. Widge , Baoshan Zhang , Mario Roederer , Julie E. Ledgerwood , Tracy J. Ruckwardt , Martin R. Gaudinski , Penny L. Moore , Nicole A. Doria-Rose , Ralph S. Baric , Barney S. Graham , Adrian B. McDermott , Daniel C. Douek , Peter D. Kwong , John R Mascola , Nancy J. Sullivan , John Misasi
期刊：Science
发表时间：2021/07/01
数字识别码：10.1126/science.abh1766
摘要：The emergence of highly transmissible SARS-CoV-2 variants of concern (VOC) that are resistant to therapeutic antibodies highlights the need for continuing discovery of broadly reactive antibodies. We identify four receptor-binding domain targeting antibodies from three early-outbreak convalescent donors with potent neutralizing activity against 23 variants including the B.1.1.7, B.1.351, P.1, B.1.429, B.1.526 and B.1.617 VOCs. Two antibodies are ultrapotent, with sub-nanomolar neutralization titers (IC50 0.3 to 11.1 ng/mL; IC80 1.5 to 34.5 ng/mL). We define the structural and functional determinants of binding for all four VOC-targeting antibodies and show that combinations of two antibodies decrease the in vitro generation of escape mutants, suggesting their potential in mitigating resistance development.
[1] Lingshu Wang et al., (2021), Ultrapotent antibodies against diverse and highly transmissible SARS-CoV-2 variants, Science, DOI: 10.1126/science.abh1766
