论文
论文标题：Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade
作者：Sneha Berry , Nicolas A. Giraldo , Benjamin F. Green , Tricia R. Cottrell , Julie E. Stein , Elizabeth L. Engle , Haiying Xu , Aleksandra Ogurtsova , Charles Roberts , Daphne Wang , Peter Nguyen , Qingfeng Zhu , Sigfredo Soto-Diaz , Jose Loyola , Inbal B. Sander , Pok Fai Wong , Shlomit Jessel , Joshua Doyle , Danielle Signer , Richard Wilton , Jeffrey S. Roskes , Margaret Eminizer , Seyoun Park , Joel C. Sunshine , Elizabeth M. Jaffee , Alexander Baras , Angelo M. De Marzo , Suzanne L. Topalian , Harriet Kluger , Leslie Cope , Evan J. Lipson , Ludmila Danilova , Robert A. Anders , David L. Rimm , Drew M. Pardoll , Alexander S. Szalay , Janis M. Taube
期刊：Science
发表时间：2021/06/11
数字识别码：10.1126/science.aba2609
摘要：Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this “AstroPath” whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti–programmed cell death-1 (PD-1)–based therapy, including CD163+PD-L1– myeloid cells and CD8+FoxP3+PD-1low/mid T cells. These features were combined to stratify long-term survival after anti–PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.
